Round: 25 Ground: Clay
| ITF Women Prague | 2016/08/28 22:00 | Alica Rusova v Aleksandra Buczynska | 3-6,7-6,4-6 |
| ITF Women Prague | 2016/08/27 22:00 | Aleksandra Buczynska v Katsiaryna Skachkova | 7-5,6-2 |
| ITF Women Prague | 2016/08/28 22:00 | Sonja Krtenova v Klara Hajkova | 7-5,3-6,4-6 |
| ITF Women Prague | 2016/08/27 22:00 | Jana Boubelikova v Klara Hajkova | 4-6,4-6 |